Juxtacellular Labeling of Individual Neurons In Vivo: From Electrophysiology to Synaptology
نویسندگان
چکیده
This chapter summarizes details pertaining to the extracellular recording and juxtacellular labeling method and its application to the characterization of basal forebrain neurons. Juxtacellular labeling is compared to other single-cell labeling techniques. Compatibility of the method with fluorescent retrograde/anterograde neural tracing, immunohistochemical, and electron microscopy techniques is also illustrated.
منابع مشابه
Electrophysiological recordings from behaving animals--going beyond spikes.
Most of our current knowledge about the neural control of behavior is based on electrophysiology. Here we review advances and limitations of current electrophysiological recording techniques applied in behaving animals. Extracellular recording methods have improved with respect to sampling density and miniaturization, and our understanding of the nature of the recorded signals has advanced. Jux...
متن کاملNoisy Juxtacellular Stimulation In Vivo Leads to Reliable Spiking and Reveals High-Frequency Coding in Single Neurons.
Single cells in the motor and somatosensory cortex of rats were stimulated in vivo with broadband fluctuating currents applied juxtacellularly. Unlike the DC current steps used previously, fluctuating stimulation currents reliably evoked spike trains with precise timing of individual spikes. Fluctuating currents resulted in strong cellular responses at stimulation frequencies beyond the inverse...
متن کاملFrom Art to Engineering? The Rise of In Vivo Mammalian Electrophysiology via Genetically Targeted Labeling and Nonlinear Imaging
F or close to half a century, neurophysiologists have been able to record electrical signals from the millions of individual neurons that compose even the smallest mammalian brain. Despite this long history, which has led to signifi cant strides toward understanding how neuronal activity translates into brain function, much of the way electrophysiological data is gathered is more of an art form...
متن کاملRecording, labeling, and transfection of single neurons in deep brain structures
Genetic tools that permit functional or connectomic analysis of neuronal circuits are rapidly transforming neuroscience. The key to deployment of such tools is selective transfection of target neurons, but to date this has largely been achieved using transgenic animals or viral vectors that transduce subpopulations of cells chosen according to anatomical rather than functional criteria. Here, w...
متن کاملSparse but selective and potent synaptic transmission from the globus pallidus to the subthalamic nucleus.
The reciprocally connected GABAergic globus pallidus (GP)-glutamatergic subthalamic nucleus (STN) network is critical for voluntary movement and an important site of dysfunction in movement disorders such as Parkinson's disease. Although the GP is a key determinant of STN activity, correlated GP-STN activity is rare under normal conditions. Here we define fundamental features of the GP-STN conn...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2005